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KMID : 0357419960260010059
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Korean journal of Virology
1996 Volume.26 No. 1 p.59 ~ p.66
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Dot Blot Assay for Screening of Anti-hantavirus Antibodies by Using Nucleocapsid Protein of Hantaan Virus
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조해월
정연준/김정림/반상자/남재환/이형우/이유진/김은정
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Abstract
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For easy and rapid screening of hemorrhagic fever with renal syndrome (HFRS) without any laboratory equipment, dot blot enzyme immunoassay was developed and tried to detect anti-hantavirus antibodies. The nucleocapsid protein of Hantaan virus was
isolated by affinity chromatography and used for making the dot strip. 28 of 29 Hantaan virus infected sera showed positive signals and 21 of 22 HFRS negative sera showed no Positive signals. Anti-Seoul virus monoclonal antibody also exibited
positive
signal but the intensity of colorization was
approximately 5 fold less than that of anti-Hantaan monoclonal antibody, The sensitivity of dot blot assay was equal or superior to indirect immunofluorescent assay (IFA) or ELISA test. Overall, the screening results with dot blot assay showed
92.2% of
concordance with IFA or ELISA test. This results suggests that dot blot assay could be applied a tool for easy and rapid screening of HFRS.
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